Anti-SIN3B Antibody

This rabbit polyclonal antibody targets SIN3B, a transcriptional regulatory protein that acts as a core component of the SIN3 histone deacetylase complex and contributes to chromatin remodeling and gene silencing. Produced by Antibodies.com, this unconjugated IgG reagent is validated for Western blot detection of endogenous SIN3B in samples derived from human, mouse, and rat origin, supporting comparative studies of epigenetic regulation across species and tissue types.

Supplied as 50 µl of polyclonal antiserum, the antibody is suited to probing SIN3B expression in whole-cell or tissue lysates prepared for immunoblotting workflows. The unconjugated format leaves secondary detection choices open, allowing flexible pairing with HRP-conjugated anti-rabbit IgG for chemiluminescence imaging or with fluorophore-labeled secondaries for multiplex fluorescence readout. Researchers investigating chromatin-modifying complexes, cell cycle progression, differentiation programs, or DNA damage response pathways can use this reagent to evaluate SIN3B protein abundance under varied experimental conditions.

For handling, the antibody should be stored refrigerated at 4 °C for short-term use; aliquoting and freezing at -20 °C or below is recommended for extended storage to limit freeze-thaw cycles and preserve activity. Protect from light when the preparation will subsequently be combined with fluorescent secondaries. Each lot is QC-tested by Western blot to confirm band pattern consistent with the predicted molecular weight of SIN3B in relevant cell lysates.