Anti-SIN3B Antibody

This rabbit polyclonal antibody from Antibodies.com selectively targets SIN3B, a scaffold subunit of the SIN3A/SIN3B histone deacetylase complex involved in chromatin remodeling, gene silencing, and cell cycle control. The unconjugated IgG reagent is supplied as a 100 µL vial and is validated for Western blot detection across human, mouse, and rat samples.

Investigators studying the SIN3B tumor suppressor function, particularly in breast cancer and hepatocellular carcinoma where the protein regulates cyclin-dependent kinase inhibitors, use this reagent to probe endogenous expression. The polyclonal format recognizes multiple epitopes on SIN3B, which is advantageous when examining isoforms or post-translational modifications. The reagent also supports research into DNA damage response, neuronal differentiation, and the pluripotency network where SIN3B interacts with NANOG and OCT4.

For storage, freeze at -20 °C in working aliquots to limit freeze-thaw cycling, and the unconjugated format allows researchers to conjugate the antibody to fluorophores, HRP, or biotin as downstream detection requires. A typical working dilution for Western blot falls in the 1:500 to 1:2000 range, though optimization per sample is recommended. This product is intended for research use only.