Anti-SIAH1 Antibody

Raised in rabbit, this unconjugated polyclonal antibody recognizes SIAH1, an E3 ubiquitin-protein ligase containing a RING finger domain that targets cellular substrates for proteasomal degradation. Supplied as 100 µl of liquid reagent, it is validated for western blot and immunohistochemistry workflows on human samples, supporting studies of apoptosis regulation, hypoxia signaling, DNA damage response, and tumor suppressor pathways.

SIAH1 mediates ubiquitination of diverse substrates including TRAF2, DCC, and PML, linking the ligase to programmed cell death, neuronal development, and oxidative stress responses. Investigators apply this reagent to map how SIAH1-driven proteasomal turnover shapes p53-dependent checkpoints and oncogenic signaling cascades in cancer cell models. The unconjugated format permits flexible pairing with HRP, fluorophore, or biotin secondary reagents, while the rabbit host suits standard anti-rabbit detection chemistries in both chromogenic and fluorescent IHC protocols.

Each vial provides sufficient material for multiple blotting membranes or tissue section experiments. Documentation from Antibodies.com confirms reactivity with human SIAH1 epitopes and lists applications as WB and IHC. Store refrigerated upon arrival, protect from light, avoid repeated freeze-thaw cycles, and aliquot if extended use over several weeks is planned. Researchers targeting E3 ligase biology, ubiquitin-mediated proteolysis, or stress-activated apoptosis pathways will find this reagent a practical addition to their antibody panel.