Anti-SIAH1 Antibody

Primary antibodies raised against the SIAH1 protein enable investigation of this E3 ubiquitin-protein ligase across multiple experimental systems. The polyclonal reagent from Antibodies.com recognizes human, mouse, and rat SIAH1 and is supplied as 100 µl of unconjugated antibody validated for western blot, immunohistochemistry, and immunofluorescence workflows.

SIAH1 (Seven In Absentia Homolog 1) operates within the ubiquitin-proteasome system, catalyzing ubiquitin transfer onto substrate proteins such as PHD3, PML, TRAF2, and components of the p53 apoptosis pathway. The ligase has documented roles in hypoxia response signaling, neuronal differentiation, and DNA damage responses, making it a target of interest in tumor suppressor research, neurodegeneration studies, and investigations of hypoxic gene regulation.

Researchers use this reagent to probe endogenous SIAH1 expression, subcellular localization, and interaction partners in cultured cell lines and primary tissue. The unconjugated format allows flexible pairing with secondary antibodies conjugated to HRP, fluorophores, or biotin, supporting both chromogenic and fluorescence detection strategies. Store refrigerated and avoid repeated freeze-thaw cycles to preserve binding activity across experiments.