Anti-Shc1 (Ab-427) Antibody

Antibodies.com offers a rabbit polyclonal antibody raised against Shc1, specifically recognising the Ab-427 epitope. Categorised as a primary antibody, the unconjugated reagent is produced in rabbit and is reactive with human samples.

Specificity is directed at a peptide sequence around amino acids 425–429 (P-S-Y-V-N) of human Shc1, allowing detection of endogenous levels of total Shc1 protein. The antibody is validated for use in Western blotting (WB), immunohistochemistry (IHC) and immunocytochemistry/immunofluorescence (ICC/IF), and is supplied at 1.0 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol. It sits within a broader Antibodies.com portfolio of 16 SHC monoclonal and polyclonal antibodies validated across WB, IHC, ICC/IF, ELISA and IP.

Polyclonal antibodies were generated by immunising rabbits with synthetic peptide conjugated to KLH, then purified by affinity chromatography using the epitope-specific peptide. The product is shipped at 4 °C; upon receipt it should be aliquoted and stored at −20 °C, with repeated freeze/thaw cycles avoided. The reagent is supported by 3 scientific validation images.