Anti-SH3KBP1 Antibody

This polyclonal antibody from Antibodies.com targets SH3KBP1, also known as CIN85 or Ruk, a multifunctional adaptor protein that operates at the intersection of clathrin-mediated endocytosis, receptor downregulation, and ubiquitin-dependent signaling through Cbl family ligases. Raised in rabbit and affinity-purified, the reagent is supplied as 100 µl of unconjugated antiserum in a single vial, validated for Western blot and immunohistochemistry on human tissue and cell-derived samples.

SH3KBP1 functions as a molecular scaffold that couples activated receptor tyrosine kinases to the endocytic machinery and remodels the actin cytoskeleton, with documented roles in apoptosis regulation, podosome assembly, and immune cell receptor signaling. Because CIN85 engages numerous binding partners through its three SH3 domains, a reagent recognizing the endogenous protein supports studies of EGFR trafficking, T-cell and B-cell receptor turnover, and stress-activated kinase cascades relevant to cancer and inflammation research.

For Western blot, titrate the antibody to determine the optimal working dilution and pair it with an appropriate HRP- or fluorophore-conjugated secondary for chemiluminescent or fluorescent detection. For immunohistochemistry, perform antigen retrieval in citrate buffer prior to overnight incubation on formalin-fixed paraffin-embedded sections. Aliquot upon first thaw, store at -20°C in working volumes, and avoid repeated freeze-thaw cycles to preserve binding activity. Intended for research use only.