Anti-SH3GLB2 Antibody

Primary Antibodies from Antibodies.com include this affinity-purified rabbit polyclonal reagent raised against SH3GLB2, also known as Endophilin B2, a SH3-domain GRB2-like protein that localizes to intracellular membranes and participates in autophagy regulation, mitochondrial outer membrane permeabilization, and Bax-mediated apoptotic signaling. The IgG antibody is supplied as a 100 µl vial of unconjugated liquid in PBS (pH 7.4) with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant, and it recognizes total endogenous SH3GLB2 migrating near 43 kDa on Western blot membranes.

Validated for Western blot and immunohistochemistry on human, mouse, and rat samples, the reagent supports comparative work across in vitro cell lysates and in vivo tissue sections. Recommended starting antibody dilutions of 1:500–1:1000 for immunoblotting and 1:50–1:100 for IHC accommodate most standard chemiluminescent and chromogenic detection workflows. Because SH3GLB2 interacts with Beclin 1 and other endophilin-family partners, the antibody is useful for subcellular localization studies, mitochondrial dynamics research, and apoptosis pathway profiling in cancer biology, neuroscience, and endosomal trafficking investigations.

For handling, aliquot the antibody upon arrival and store at -20°C to minimize freeze-thaw cycles; refrigerated working solutions remain stable for short-term use. The unconjugated format allows direct labeling with fluorophores, biotin, or HRP to match individual experimental needs, and each batch is affinity-purified against the immunizing peptide for reproduciblelot-to-lot specificity in downstream Western blot and IHC workflows.