Anti-SGK1 Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against serum/glucocorticoid-regulated kinase 1 (SGK1) and is supplied as an unconjugated, liquid antiserum-derived preparation in PBS containing 0.1% sodium azide and 50% glycerol at a concentration of 1 mg/mL. The 50 µL vial delivers a polyclonal IgG antibody validated for Western Blotting (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) applications, with reactivity confirmed against human, mouse, and rat samples. The immunogen corresponds to a recombinant fusion protein spanning amino acids 260–431 of human SGK1 (NP_005618.2), allowing the reagent to detect endogenous levels of the kinase across multiple species. Recommended working dilutions are WB1:500–1:1,000, IHC 1:50–1:200, and ICC/IF 1:50–1:200, with the expected molecular weight band falling in the 45–60 kDa range. For routine use, the antibody should be stored at –20 °C in single-use aliquots to avoid repeated freeze/thaw cycles, and gentle mixing is advised before each application to preserve affinity-purified performance.