Anti-SGCG Antibody

Raised in rabbit and validated for human samples, this polyclonal IgG reagent from Antibodies.com binds gamma-sarcoglycan (SGCG), the transmembrane glycoprotein that anchors the dystrophin-associated protein complex to the sarcolemma of skeletal and cardiac muscle. Loss-of-function mutations in the SGCG gene on chromosome 13q12 cause limb-girdle muscular dystrophy type 2C, making this antibody a useful probe for characterizing disease-associated protein variants in patient biopsies and cellular models.

The unconjugated primary antibody is supplied as 200 µL of liquid, ready to dilute into standard Western blot workflows. Because the polyclonal response recognizes multiple epitopes, the reagent tolerates modest structural variation across truncated or mutant forms of the target, supporting detection of endogenous gamma-sarcoglycan in muscle lysates alongside loading controls such as vinculin or alpha-tubulin. Each lot is confirmed to react with the human antigen and shows no significant cross-reactivity with mouse or rat in routine validation, allowing clear interpretation of orthologous comparison studies when paired with a species-specific secondary antibody.

Researchers typically dilute the stock 1:500 to 1:2000 for immunoblotting of whole-cell or tissue extracts, with optimization depending on sample abundance. Store the vial refrigerated at 4°C for short-term use, and aliquot on first opening to minimize repeated freeze-thaw cycles when storing at -20°C for longer projects. Single-use aliquots preserve antibody integrity across multi-week experimental timelines common to dystrophinopathy research.