Anti-SFRP4 Antibody

This polyclonal antibody from Antibodies.com targets SFRP4 (Secreted Frizzled-Related Protein 4), a soluble modulator of the Wnt/β-catenin signaling pathway studied in contexts ranging from bone metabolism and adipocyte biology to epithelial tumor progression. Raised in rabbit and supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated reagent is validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples. Each vial contains 100 µl of the primary antibody, enough for titration across multiple membranes or tissue sections. Investigators working on colorectal, endometrial, ovarian, or renal carcinoma models have used anti-SFRP4 reagents to assess loss of Wnt pathway inhibition, while metabolic researchers employ the antibody to probe SFRP4 expression in adipose tissue and pancreatic islets relevant to insulin resistance. The polyclonal format increases the likelihood of detecting multiple epitopes on the secreted glycoprotein, which helps when working with truncated splice variants or partially degraded lysates. Storage at -20°C in single-use aliquots is recommended to limit freeze-thaw cycles of the glycerol-containing buffer; protect from light and avoid vortexing to preserve antibody integrity. For research use only.