Anti-SFRP2 Antibody

Manufactured by Antibodies.com, this polyclonal reagent is raised in rabbit and validated for the detection of SFRP2, a secreted frizzled-related protein implicated in Wnt signalling and extracellular matrix biology. The antibody is supplied as a 50 µl aliquot at a concentration of 1.0 mg/mL in phosphate-buffered saline (pH 7.4) with 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a cryoprotectant. The host species is rabbit, the clonality is polyclonal, and the reagent is unconjugated, giving users full flexibility to choose their own secondary label or detection chemistry. It has been affinity-purified against a recombinant human SFRP2 immunogen, ensuring specificity for the endogenous protein. Recommended applications include Western blotting at 1:500 to 1:2000 and immunofluorescence at 1:50 to 1:100, with representative validation data shown on HeLa cells by IF and on paraffin-embedded rat brain tissue. The product is shipped at 4°C and should be aliquoted and stored at -20°C upon arrival, with repeated freeze/thaw cycles avoided to preserve activity. Intended for research use only, this Antibodies.com reagent provides a reliable tool for laboratories investigating SFRP2 function across mouse and related mammalian model systems.