Anti-SF3B3 Antibody

Raised against the spliceosomal SF3B3 subunit, this unconjugated rabbit polyclonal antibody from Antibodies.com is validated for western blot detection of Splicing Factor 3b Subunit 3 across human, mouse, and rat samples. Supplied at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the reagent is packaged as a 50 µl vial ready for immunoblotting experiments targeting the U2 small nuclear ribonucleoprotein complex.

SF3B3 serves as the largest component of the SF3b complex within U2 snRNP, contributing to branch site recognition during spliceosome assembly and catalytic activation of pre-mRNA splicing. Researchers studying RNA processing, alternative splicing regulation, spliceosomal assembly kinetics, or cancer-associated splicing dysregulation use this reagent to probe endogenous SF3B3 expression in whole cell lysates and tissue homogenates. The polyclonal format recognizes multiple epitopes on the target, supporting strong signal in chemiluminescent western blot workflows and offering flexibility for downstream applications such as immunoprecipitation or co-immunoprecipitation experiments involving splicing factor interactors.

For best results, store the vial refrigerated; the sodium azide preservative maintains antibody stability for extended use. Aliquot upon first thaw to minimize freeze-thaw cycles, and handle using standard cold-chain practices during routine bench work.