Anti-SF3B3 Antibody

A rabbit polyclonal IgG primary antibody from Antibodies.com, supplied as 50 µl of unconjugated polyclonal antiserum, supports detection of endogenous SF3B3 in human, mouse, and rat samples. The approximately 135 kDa target, also known as SAP130, is a subunit of the U2 small nuclear ribonucleoprotein that participates in spliceosome assembly and pre-mRNA splicing.

The antibody is validated for western blot, immunohistochemistry, and immunofluorescence. In western blot workflows with cell lysates, it can help assess changes in SF3B3 abundance during studies of RNA processing or spliceosome function. Immunohistochemistry enables localization in fixed tissue sections, while immunofluorescence supports imaging of SF3B3 in labeled cell preparations. These applications support investigations into U2 snRNP biogenesis, spliceosome assembly, and cellular RNA-processing pathways across the listed species.

Its unconjugated format allows pairing with a compatible labeled secondary reagent selected for the assay. Store the vial at -20 °C in working aliquots and limit freeze-thaw cycles to preserve antibody activity. This reagent is for research use only.