Anti-SF3B1 Antibody

Raised in rabbit as a polyclonal reagent, this unconjugated antibody recognises SF3B1 (Splicing Factor 3b Subunit 1), a core component of the spliceosomal U2 small nuclear ribonucleoprotein complex responsible for 3' splice site recognition during pre-mRNA splicing. Supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, the 50µl format from Antibodies.com is validated for Western blot applications on lysates from human, mouse, and rat samples. SF3B1 is a focal target in RNA splicing research, spliceosome assembly studies, and investigations into the recurrent SF3B1 hotspot mutations that drive myelodysplastic syndromes, chronic lymphocytic leukaemia, uveal melanoma, and certain breast cancers. The unconjugated format allows flexible pairing with HRP or fluorescent secondary reagents for chemiluminescent or fluorescence-based detection of endogenous SF3B1 protein levels in whole-cell extracts and tissue homogenates.