Anti-SF1 (Ab-82) Antibody

Manufactured by Antibodies.com, this primary antibody is a rabbit polyclonal reagent raised against splicing factor 1 (SF1), specifically directed at the region surrounding the serine 82 phosphorylation site (immunogen: synthesized non-phosphopeptide derived from human SF1 around S-P-S(p)-P-E). The antibody is supplied as an unconjugated, affinity-purified IgG fraction at a concentration of 1 mg/ml, formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. The 50 µl volume is intended for detection of endogenous levels of total SF1 protein in human samples, and it has been validated for use in Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Being a polyclonal, the reagent recognizes multiple epitopes on the target, which can be advantageous for robust signal generation. For long-term stability, the antibody should be stored at –20 °C; under these conditions the manufacturer supports a 100% guarantee and ships the product from Cambridge, UK, with a typical lead time of 6–9 business days. It is recommended for research use only and is not intended for diagnostic or therapeutic applications. This reagent is suitable for laboratories investigating RNA splicing, post-translational regulation of SF1 at Ser82, and related signal-transduction pathways in human cell and tissue models.