Anti-SF1 (Ab-82) Antibody

SF1 (Splicing Factor 1) is a nuclear protein essential for early spliceosome assembly, recognizing the branch point sequence during pre-mRNA splicing. This unconjugated rabbit polyclonal reagent from Antibodies.com targets an epitope surrounding residue 82 of human SF1, supporting detection of the endogenous protein in research samples.

The antibody is validated for western blot, immunohistochemistry, and immunofluorescence, covering both denaturing and native workflows across fixed cells and tissue sections. Supplied as 100 µl of polyclonal serum raised in rabbit, the reagent is intended for research use only, with reactivity confirmed against human SF1.

SF1 has been characterized in steroidogenic and reproductive tissues, where its dual role in early spliceosome assembly and transcriptional co-regulation links RNA processing to nuclear receptor signaling. For long-term storage, keep refrigerated and aliquot upon receipt to avoid repeated freeze-thaw cycles that can erode polyclonal titer over time. The unconjugated format allows researchers to pair the primary antibody with custom fluorophore or enzyme conjugates of their choice.