Anti-SETD3 Antibody

SETD3 is a SET-domain-containing histidine methyltransferase best known for methylating β-actin at histidine 73, a modification that influences cytoskeletal dynamics, cell migration, and contractile function in muscle tissue. Rabbit polyclonal antibodies against SETD3 are widely used to probe these cellular roles and to investigate links between SETD3 activity and glucose homeostasis, since the enzyme regulates glucose uptake in myocytes. Researchers have also deployed SETD3-targeting antibodies in studies of viral replication, where the methyltransferase supports the life cycle of several enteroviruses.

This unconjugated preparation from Antibodies.com is supplied as 100 µl of affinity-purified polyclonal IgG, validated for Western blot (WB) and immunoprecipitation (IP) workflows on human and mouse samples. It is offered in the Primary Antibodies category and works on whole-cell lysates or tissue homogenates from cultured mammalian cells and animal models, where detection of a single band at the expected molecular weight indicates specific recognition of endogenous SETD3. The unconjugated IgG format allows flexible pairing with HRP, fluorophore, or biotin-conjugated secondary reagents in custom detection workflows.

For best results, refrigerate the antibody upon arrival, prepare small working aliquots to limit repeated freeze-thaw cycles, and centrifuge briefly before use to recover the full volume. Apply standard dilution ranges commonly used for rabbit polyclonal reagents (typically 0.5-2 µg/ml for WB), adjusting as needed for target abundance and detection chemistry in each assay.