Anti-SET Antibody

The SET nuclear oncoprotein, also referred to as I2PP2A or TAF-Iβ, is a multifunctional histone chaperone and a potent endogenous inhibitor of protein phosphatase 2A that participates in chromatin remodeling, transcription regulation, and suppression of apoptosis. Antibodies.com supplies the reagent as an affinity-purified rabbit polyclonal antibody at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, packaged as a 100 µl aliquot and validated for western blot and immunohistochemistry on human, mouse, and rat samples.

The antibody is raised against endogenous SET and detects the canonical isoforms that migrate at approximately 33 kDa by SDS-PAGE, distributing between nucleus and cytoplasm in a stimulus-dependent manner. Researchers working on SET-NUP214-driven leukemogenesis, PP2A reactivation strategies in cancer, or histone chaperone dynamics use this reagent to follow SET expression in cultured cell lines, primary tissue sections, and whole-cell lysates. The polyclonal format broadens epitope coverage compared with a single monoclonal clone, which is useful when SET is being tracked across different post-translational states or in rodent orthologs.

For storage, the glycerol-containing buffer is intended to be held at -20 °C, with aliquoting recommended to avoid repeated freeze-thaw cycles; the 0.1% sodium azide preservative permits short-term handling on ice for routine western blot and IHC workflows. The unconjugated format gives users flexibility to couple the antibody to their preferred reporter or bead chemistry.