Anti-SERPINC1 Antibody

Antithrombin III, the protein product of the SERPINC1 gene, is a circulating serine protease inhibitor that inactivates thrombin and factor Xa through slow tight-binding kinetics, anchoring the regulation of the coagulation cascade. Antibodies.com provides a rabbit polyclonal anti-SERPINC1 antibody supplied as 100 µl of a 1 mg/ml stock in phosphate buffered saline (PBS) with 15 mM sodium azide at approximately pH 7.2, unconjugated and ready for direct labeling or downstream detection workflows.

Validated for western blot detection, this polyclonal reagent recognizes SERPINC1 from human, mouse, and rat samples, making it a useful tool for comparative studies of hepatic synthesis, plasma clearance, and disease-associated variants of antithrombin. The unconjugated format offers flexibility in choosing HRP, biotin, or fluorophore-conjugated secondary reagents depending on the application. Sodium azide at 15 mM is included as a preservative; for activity-sensitive assays or planned conjugation reactions, it should be removed by buffer exchange prior to use.

For long-term storage, aliquot the antibody and store at -20°C, avoiding repeated freeze-thaw cycles to preserve antibody activity. Short-term working aliquots may be kept refrigerated at 4°C for several weeks. The 100 µl volume provides ample reagent for multiple western blot experiments across mini or midi gel formats and supports re-probing of membranes for normalization against housekeeping proteins.