Anti-SEMA3A Antibody

Generated in rabbit and supplied as an IgG preparation, this unconjugated polyclonal reagent from Antibodies.com is raised against Semaphorin 3A (SEMA3A), a secreted guidance cue that signals through neuropilin-1 and plexin-A receptor complexes to direct axon pathfinding and modulate immune cell activity.

The 50 µl aliquot is validated for Western blot detection of endogenous SEMA3A in lysates prepared from human and mouse samples. Because the antibody is polyclonal, it recognizes multiple epitopes on the target, which is helpful when expression levels vary between cell types, developmental stages, or tissue origins. An HRP-conjugated secondary reagent is used for visualization after SDS-PAGE separation and membrane transfer.

SEMA3A is studied in neuroscience as a chemorepellent for growing axons, in immunology for its role in T-cell and dendritic-cell regulation, and in oncology, where altered expression has been reported in tumor microenvironment studies. The reagent's cross-reactivity with both human and mouse SEMA3A makes it useful for translational work that bridges cell-line models and primary tissue. For storage, keep refrigerated and protect from repeated freeze-thaw cycles to preserve antibody activity.