Anti-SEK1 / MKK4 (Ab-80) Antibody

This rabbit polyclonal antibody from Antibodies.com targets SEK1, also known as MKK4 or MAP2K4, a dual-specificity kinase positioned at a critical junction of stress-activated MAPK signaling cascades. The reagent is supplied unconjugated in a 100 µl vial and is validated for Western blot and immunohistochemistry workflows across Human, Mouse, and Rat samples, supporting reproducible detection of endogenous SEK1/MKK4 in cell lysates and fixed tissue sections.

SEK1/MKK4 integrates upstream signals from MEKK1 and other MAP3Ks to phosphorylate JNK and p38 family kinases, making it a relevant reagent for studies of inflammatory signaling, oxidative stress responses, and apoptosis pathways. Polyclonal rabbit immunisation yields recognition of multiple epitopes on the target, an advantage when monitoring expression changes or isoform distribution under varied experimental conditions. Researchers deploy this antibody to probe SEK1/MKK4 levels in signal transduction work, where reliable cross-species reactivity in human, murine, and rat model systems broadens experimental design flexibility.

The unconjugated format allows the user to couple the antibody to HRP, biotin, fluorophores, or other labels according to the secondary detection chemistry required for a given workflow. For routine bench use, store refrigerated and protect from light to preserve reagent activity across repeated experiments.