Anti-SDHB Antibody

This rabbit polyclonal antibody recognizes the iron-sulfur subunit B (SDHB) of mitochondrial succinate dehydrogenase (Complex II), a key enzyme bridging the TCA cycle and the electron transport chain. Raised in rabbits and supplied as an unconjugated reagent at 100 µl per vial, it is validated for Western blot and immunohistochemistry on human and mouse samples, making it a practical tool for studying mitochondrial metabolism and SDHB-linked tumor biology.

SDHB loss-of-function mutations carry clear clinical relevance: they underlie hereditary paraganglioma-pheochromocytoma syndromes and are also observed in SDH-deficient gastrointestinal stromal tumors and renal cell carcinomas. Researchers routinely apply the antibody to evaluate SDHB expression in tumor tissue, where absent staining supports the classification of SDH-deficient neoplasms. The polyclonal format increases the likelihood of detecting the target even when sample handling has partially compromised antigen integrity.

For Western blot, the antibody recognizes the expected band near 32 kDa under reducing conditions. For IHC, antigen retrieval with citrate buffer at pH 6.0 is recommended on formalin-fixed paraffin-embedded sections. Store the vial refrigerated at 4 °C; aliquot upon first use and avoid repeated freeze-thaw cycles to preserve activity over the working shelf life.

Provided by Antibodies.com, each lot is quality-controlled to support reproducible results across basic and translational research workflows.