Anti-SAP130 Antibody

Raised in rabbit and supplied as an unconjugated polyclonal antiserum from Antibodies.com, this 100 µl vial targets SAP130 (Sin3A-associated protein 130), a ~130 kDa subunit of the spliceosomal SF3b complex that localizes to nuclear speckles and contributes to pre-mRNA splicing. The antibody is validated for Western blot and immunohistochemistry on human samples, where it detects the endogenous nuclear protein in cell lysates and fixed tissue sections. Researchers studying splicing factor composition, chromatin-associated complexes, or hepatocellular carcinoma biology — where altered SAP130 expression has been reported — will find this reagent useful for confirming protein levels and subcellular localization. The polyclonal format increases the likelihood of recognizing multiple epitopes on the target, which can be helpful when probing denatured samples in immunoblots. Storage and handling recommendations typically include aliquoting to limit freeze-thaw cycles and protecting the reagent from light to preserve antibody activity over the product shelf life.