Anti-S100B Antibody

Target the S100 calcium-binding protein B with this unconjugated rabbit polyclonal reagent from Antibodies.com, supplied as a 50 µl vial at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol. Raised against the S100B antigen, the antibody is validated for Western Blot and Immunohistochemistry across Human, Mouse, and Rat samples, supporting studies of astrocyte and Schwann cell biology as well as melanoma biomarker research where S100B serves as a clinically relevant marker. The polyclonal response targets multiple epitopes on the S100B monomer (~10.5 kDa) and its ~21 kDa homodimer, allowing reliable detection in denatured protein lysates and fixed tissue sections alike. Typical working dilutions are 1-5 µg/ml for Western blotting and 1:100-1:500 for IHC on paraffin-embedded or cryosectioned tissue. Store the vial at -20 °C in its glycerol-containing buffer; the 50% glycerol cryoprotectant prevents ice crystal formation, so aliquoting before freezing is recommended to limit repeated freeze-thaw cycles and preserve long-term activity.