Anti-S100A4 Antibody

Targeting the calcium-binding S100 family protein implicated in epithelial-mesenchymal transition and metastatic progression, this polyclonal reagent is supplied by Antibodies.com as a rabbit host antibody for Western blot and immunohistochemistry workflows. Validated reactivity across human, mouse, and rat orthologs makes it appropriate for cross-species studies of the tumor microenvironment, stromal fibroblast labeling, and EMT status confirmation in cell-based assays. The unconjugated antibody is provided at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, packaged as 100 µl per vial to support multiple gel and slide staining runs. Polyclonal recognition of multiple epitopes aids detection of conserved calcium-binding regions shared among mammalian S100A4 orthologs. The glycerol-containing formulation is stored at -20 °C and protected from light to preserve activity across repeated freeze-thaw cycles; aliquot upon first use. For research use only, this reagent is commonly deployed to identify cancer-associated fibroblasts in tumor sections, confirm mesenchymal phenotype in cell models, and detect endogenous S100A4 expression in tissue lysates.