Anti-RUVBL1 Antibody

RUVBL1 (RuvB-like protein 1), also known as Pontin or TIP49a, is an ATPase of the AAA+ family that functions within chromatin-remodeling complexes such as INO80 and TIP60, where it partners with the related factor RUVBL2/Reptin to drive transcriptional regulation, ribosome biogenesis, and the DNA damage response. This polyclonal reagent from Antibodies.com is raised in rabbit and recognizes the human, mouse, and rat RUVBL1 protein, supporting detection by Western blot, immunohistochemistry on paraffin and frozen sections, and immunofluorescence microscopy in cell culture and tissue specimens.

Provided as 100 µl of unconjugated antibody at 1 mg/ml in PBS supplemented with 0.1% sodium azide and 50% glycerol, the preparation is aliquoted for single-use experiments and tolerates standard freeze-thaw handling when stored at -20°C, with working dilutions typically prepared fresh before each immunostaining protocol. The reagent has been applied to studies of Wnt/β-catenin signaling, oncogenic transcription programs, and the assembly of ATP-dependent chromatin remodelers, where it enables probing of endogenous RUVBL1 levels in lysates and co-localization analyses alongside Reptin/RUVBL2 partners in cancer cell lines and primary samples.