Anti-RUNX2 Antibody

Antibodies.com offers a rabbit polyclonal primary antibody raised against RUNX2, supplied as an unconjugated IgG in liquid form. According to the manufacturer's datasheet for this product, it has been validated for Western blotting, immunohistochemistry, and immunocytochemistry/immunofluorescence, with reactivity demonstrated against human, mouse and rat samples.

The immunogen is a synthetic peptide corresponding to a sequence within amino acids 200-300 of human RUNX2, and the target protein has an expected molecular weight of 60 kDa. The antibody is affinity-purified and formulated in phosphate-buffered saline (pH 7.3) containing 50% glycerol and 0.05% Proclin 300. Recommended working dilutions are 1:500-1:1,000 for Western blot and 1:50-1:200 for both IHC and ICC/IF. The manufacturer recommends shipping at 4°C, aliquoting on receipt, and storing at -20°C while avoiding repeated freeze/thaw cycles.