Anti-RUNX1T1 Antibody

For Western blot studies of transcriptional regulation and hematopoietic differentiation, this rabbit polyclonal primary antibody detects RUNX1T1 in human, mouse, and rat samples. Antibodies.com supplies 50 µl of unconjugated serum with an IgG isotype, allowing researchers to select a suitable secondary antibody and detection system. The reagent can be used to probe whole-cell lysates for the endogenous approximately 67 kDa protein and to compare full-length RUNX1T1 with truncated AML1-ETO fusion products in models of the t(8;21)(q22;q22) acute myeloid leukemia subtype.

RUNX1T1, also known as ETO or MTG8, is a nuclear transcriptional co-repressor that contacts DNA-bound transcription factors and recruits N-CoR/Sin3a/histone deacetylase complexes. Its four nervy homology regions support these interactions, while alternative transcripts generate distinct protein forms. Researchers can use this antibody to examine isoform patterns, apoptosis-associated fragments, and changes in chromatin-mediated gene silencing during myeloid differentiation and leukemic transformation.