Anti-RUNX1 Antibody

This rabbit polyclonal antibody targets RUNX1 (Runt-related transcription factor 1), a key transcriptional regulator of definitive hematopoiesis and a frequent subject of study in leukemia biology, where RUNX1 loss-of-function mutations are associated with acute myeloid leukemia and with familial platelet disorder with predisposition to AML. Raised in rabbit and supplied unconjugated at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, the reagent from Antibodies.com is validated for western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and immunoprecipitation (IP) workflows. The antibody recognizes RUNX1 from human, mouse, and rat samples, supporting comparative studies across these three species in cell culture and tissue-based experiments. Each vial contains 100 µl of antibody, sufficient for repeated use across multiple applications, and the PBS-based formulation with azide provides a stable working solution for routine immunoassay protocols. Laboratory groups investigating hematopoietic stem cell biology, megakaryocyte differentiation, or the molecular pathology of RUNX1-driven leukemias can use this polyclonal reagent to detect endogenous protein in cell lysates, fixed tissue sections, and cultured cells. The unconjugated format allows flexible pairing with user-selected secondary antibodies, fluorophores, or enzyme reporters depending on the chosen detection modality.