Anti-RUFY2 Antibody

RUFY2 (RUN and FYVE domain-containing protein 2) is a multidomain scaffolding protein best known for its role in early endosome trafficking through interactions with Rab4 and Rab5 small GTPases, and it has more recently been linked to autophagy regulation and actin remodeling at the cell periphery. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com is raised against RUFY2 and has been validated for Western blot and immunohistochemistry workflows on human, mouse, and rat samples. Each vial is supplied as a 50 µL aliquot of liquid antiserum and should be stored at -20 °C in single-use portions to limit freeze-thaw cycles that can reduce titer. The polyclonal format provides broader epitope coverage than a monoclonal reagent, which is useful when probing endogenous RUFY2 levels in tissue sections or in whole-cell lysates where post-translational modification or splice variation may mask a single linear epitope. Because the target participates in endosomal sorting and autophagosome maturation, the antibody is commonly used in studies of membrane trafficking, receptor recycling, and the crosstalk between endocytic and autophagic pathways.