Anti-RUFY2 Antibody

Produced in rabbit hosts and affinity purified against the RUFY2 target, this polyclonal IgG reagent from Antibodies.com is supplied as 200 µL of unconjugated antiserum suited to both western blot and immunohistochemistry workflows on human, mouse, and rat samples. RUFY2 (RUN and FYVE domain containing 2) pairs an N-terminal RUN domain with a C-terminal FYVE zinc finger, anchoring it to phosphatidylinositol 3-phosphate enriched endosomal membranes where it coordinates early endosome trafficking, Rab GTPase signaling, and selective cargo sorting during receptor recycling. Researchers apply the antibody to track RUFY2 redistribution in migrating cancer cell lines, map its recruitment to phagosomes during bacterial uptake, and follow its expression in neuronal cultures and formalin-fixed paraffin-embedded tissue. Each vial delivers a polyclonal mixture that recognizes multiple epitopes across the full-length protein, broadening tolerance for minor sample variation between lysate preparations. Aliquot on receipt and store at -20 degrees C; protect from repeated freeze-thaw cycles to preserve activity over time. The unconjugated format leaves users free to pair the reagent with HRP, biotin, or fluorophore-labeled secondary antibodies for chromogenic, chemiluminescent, or fluorescence detection as needed. Sufficient volume is provided for multiple blotting membranes and a series of tissue sections, supporting parallel probing of RUFY2 alongside loading controls or compartment markers in cell biology and neuroscience research.