Anti-RTN4 Antibody

Raised in rabbit against a synthetic peptide region of human Reticulon-4, this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as 50 µl of liquid antibody in PBS/glycerol buffer and is validated for western blot and immunohistochemistry workflows on human, mouse, and rat samples. The polyclonal format binds multiple epitopes across the RTN4 sequence rather than a single linear determinant, which is helpful when probing the three major splice variants — Nogo-A, Nogo-B, and Nogo-C — or processed fragments generated under different lysis conditions.

RTN4 is a reticulon-family transmembrane protein best characterized in the central nervous system, where the long Nogo-A isoform acts as a myelin-associated inhibitor of neurite outgrowth after spinal cord injury and ischemic stroke. Outside the CNS, the shorter Nogo-B and Nogo-C isoforms localize to the endoplasmic reticulum membrane and shape ER tubule morphology, while RTN4 immunoreactivity is also detected at the plasma membrane of mature oligodendrocytes. This breadth of biology makes the antibody useful for projects ranging from neural regeneration and glial scar studies to ER membrane dynamics and vascular biology work, where Nogo-B has been linked to endothelial apoptosis and vascular remodeling.

Because the antibody ships unconjugated, downstream detection chemistry is left open: the primary can be paired with HRP-, biotin-, or fluorophore-conjugated secondary reagents depending on whether the workflow calls for chemiluminescent western blot, chromogenic IHC, or immunofluorescence imaging. For handling, aliquot on first use to limit freeze-thaw cycles and store refrigerated between experiments to preserve polyclonal IgG activity. Confirmed reactivity with human, mouse, and rat samples supports both standard laboratory models and cross-species comparative studies.