Anti-RSPH1 Antibody

Targeting RSPH1 in human samples, this unconjugated polyclonal antibody from Antibodies.com is supplied as 100 µl of liquid for western blot and immunohistochemistry workflows. RSPH1 is the radial spoke head component 1 protein, a structural element of motile cilia and flagella whose dysfunction is implicated in primary ciliary dyskinesia.

Because polyclonal reagents recognize several epitopes on the target, this antibody typically produces strong signal in immunohistochemistry, where preservation of native protein context is important, and works reliably in western blot applications on lysates from ciliated tissues or relevant cell lines. Researchers investigating respiratory epithelial cilia, fallopian tube ciliated cells, or developing spermatids use this reagent to probe the assembly, stability, and turnover of the radial spoke apparatus.

For each application, validate against positive and negative tissue controls and titrate the working dilution empirically, since fixation methods and antigen retrieval conditions strongly influence staining outcomes. Keep the antibody refrigerated, aliquoted to limit repeated freeze-thaw cycles, and shield it from light during downstream fluorescent detection workflows to preserve the unconjugated immunoglobulin.