Anti-RRM2B Antibody

Raised in rabbit against human RRM2B (also known as p53R2), this affinity-purified polyclonal IgG reagent from Antibodies.com targets the p53-inducible small subunit of ribonucleotide reductase that supplies dNTPs for mitochondrial DNA repair and stress-response synthesis. The unconjugated antibody is validated for Western blot detection of the endogenous ~40-41 kDa RRM2B protein in human cell and tissue lysates following SDS-PAGE separation and transfer to nitrocellulose or PVDF membranes. Each vial contains 100 µl of liquid antibody in a single-use format, supporting routine immunoblotting of the target band under reducing conditions. The rabbit host and polyclonal clonality offer flexibility in secondary reagent selection and signal amplification, which is helpful when monitoring RRM2B induction after genotoxic insult in cultured human cells and primary tissue samples. Researchers studying p53-mediated DNA damage signaling, mtDNA copy-number maintenance, and the molecular basis of mitochondrial DNA depletion syndromes can use this reagent to track RRM2B upregulation as a downstream readout of p53 pathway activation. The unconjugated format also permits downstream labeling or pairing with custom detection chemistries, enabling assay-specific optimization in DNA repair and ribonucleotide reductase research.