Anti-RRM1 Antibody

The RRM1 protein (ribonucleoside-diphosphate reductase large subunit) is the catalytic component of ribonucleotide reductase, the enzyme that supplies deoxyribonucleotide precursors for DNA synthesis. This rabbit polyclonal antibody from Antibodies.com is raised against RRM1 and supplied as 100 µl of unconjugated antiserum. It is validated for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) workflows, with cross-reactivity confirmed against human, mouse, and rat samples.

RRM1 sits at the intersection of DNA replication stress, nucleotide pool maintenance, and chemoresponse biology. In non-small cell lung cancer research, RRM1 expression levels are commonly studied as a predictive marker for gemcitabine response, and the protein's role in DNA damage repair makes it relevant to investigations of replication fork stability and dNTP supply. The polyclonal format offers multi-epitope recognition, useful when detecting a single protein across tissue sections and lysate preparations.

Storage and handling: short-term storage at 4 °C and long-term aliquoting at -20 °C helps preserve antibody activity across freeze-thaw cycles. The unconjugated format allows users to pair the antibody with their preferred secondary detection reagent (HRP, fluorophore, or biotin), supporting flexible protocol design across immunoblotting, fixed-tissue staining, and cell-based imaging experiments.