Anti-RPS6KB1 (phospho S424) Antibody

A phospho-specific polyclonal reagent from Antibodies.com, this rabbit IgG primary antibody recognises ribosomal protein S6 kinase beta-1 (RPS6KB1, also known as p70S6K) only when serine 424 carries a phosphate group, enabling researchers to monitor post-translational activation of the kinase rather than its total cellular abundance. Supplied as 50 µl of unconjugated antiserum, the antibody is validated for western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples.

p70S6K sits downstream of mTORC1, where it integrates inputs from growth factors, amino acids, and insulin to drive translation initiation and cell growth, so phospho-specific detection at S424 is most often used to confirm pathway engagement in studies that compare stimulated versus unstimulated states, or vehicle- versus inhibitor-treated cultures. The unconjugated format leaves secondary labelling (HRP, biotin, or fluorophore conjugates) to the user's preferred detection chemistry, supporting both lysate-based western blot assays and fixed-tissue or cultured-cell imaging. Aliquot on receipt to limit repeated freeze-thaw cycles and protect from light if a fluorophore conjugate is added downstream.