Anti-RPS6KB1 (phospho S424) Antibody

Phosphorylation of ribosomal protein S6 kinase B1 (RPS6KB1, also known as p70S6K) at serine 424 is an autophosphorylation event linked to activation of the mTOR signaling cascade, which governs translational control, cell growth, and metabolic responses. This rabbit polyclonal antibody from Antibodies.com specifically recognizes RPS6KB1 only when phosphorylated at S424, enabling selective analysis of kinase activation in signaling studies. Supplied as 200 µl of unconjugated IgG, the reagent is validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) workflows on human, mouse, and rat samples. Researchers studying insulin signaling, cancer cell proliferation, or translational regulation can use the antibody to monitor activation-state dynamics of this kinase across treated and untreated conditions. The polyclonal format provides robust signal amplification, and the unconjugated conjugate allows flexible secondary antibody pairing or custom fluorophore or enzyme conjugation in downstream assay development. The product is intended for research use only and should be stored according to standard antibody handling guidelines to preserve phospho-epitope integrity across freeze-thaw cycles.