Anti-RPS6 (phospho S235 + S236) Antibody

The RPS6 (phospho S235 + S236) antibody from Antibodies.com is a rabbit polyclonal reagent supplied as 200 µl of unconjugated IgG for western blot detection of ribosomal protein S6 phosphorylated at serine 235 and serine 236. Phosphorylation at these two adjacent residues is catalyzed by p70 S6 kinase (S6K1) acting downstream of mTORC1, which makes this antibody a widely used readout for studying mTOR pathway activation in cell growth, translational control, and growth factor signaling research.

The target is the ~28 kDa ribosomal protein S6, a core component of the 40S ribosomal subunit whose phosphorylation state alters the recruitment of 5' TOP mRNAs into active polysomes. In western blot applications, this antibody is used to track stimulus-dependent changes in S6K1 activity downstream of insulin, amino acids, and oncogenic signals such as mutant PI3K, Akt, or loss of TSC1/2.

Polyclonal rabbit IgG is unconjugated and validated for human samples, allowing secondary antibody selection to be tailored to detection chemistry (chemiluminescence or fluorescence). The 200 µl volume at typical working dilutions supports multiple replicate blots examining mTOR pathway activation under varying treatment conditions.