Anti-RPS6 (phospho S235 + S236) Antibody

Affinity-purified rabbit polyclonal reagent from Antibodies.com that selectively recognises ribosomal protein S6 (RPS6) only when doubly phosphorylated at serines 235 and 236. These adjacent phospho-sites are direct substrates of p70 S6 kinase (S6K1) within the mTORC1 signalling cascade, so the antibody serves as a sensitive read-out of pathway activation in studies of translational control, cell growth, autophagy and oncogenic signalling. The polyclonal IgG backbone, raised against a synthetic phospho-peptide, presents multiple epitope specificities around the modification, which is useful when comparing lysates where phosphorylation stoichiometry varies between preparations.

Validated for Western blot on human samples, the reagent integrates with standard chemiluminescent, HRP and near-infrared detection workflows. Common experimental contexts include monitoring mTOR pathway activity in response to small-molecule inhibitors or growth factor stimulation, characterising downstream signalling in cultured cell lines, and profiling tumour specimens with dysregulated ribosomal protein phosphorylation. Because phospho-specific antibodies lose activity with repeated freeze-thaw cycles, aliquoting upon arrival and refrigerated storage are recommended to preserve titre across multiple experiments.

The antibody is supplied unconjugated in a 100 µl vial, giving researchers flexibility to pair it with a secondary reagent matched to their preferred detection chemistry, whether HRP-conjugated, fluorescently labelled, or biotinylated. The unconjugated format also enables custom immunoassay development where preservation of the phosphorylation state is essential. This product from Antibodies.com is intended for research use only and is not validated for diagnostic or therapeutic applications.