Anti-RPS6 (phospho-S235) Antibody

This polyclonal antibody targets ribosomal protein S6 phosphorylated at serine 235 (RPS6 phospho-S235), a downstream marker of mTORC1/p70 S6 kinase signaling that reflects active translation initiation and ribosome biogenesis. Manufactured by Antibodies.com, the reagent is supplied as 100 µl of unconjugated IgG at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, ready for direct labeling or use in standard detection workflows. Validated applications include Western blot for tracking pathway activation across time-course or treatment studies, and immunofluorescence for visualizing phosphorylated S6 distribution in fixed cells and tissue sections. The antibody shows reactivity with human, mouse, and rat samples, making it useful for translational research spanning cell lines, primary cultures, and rodent disease models. It is commonly deployed in studies of growth factor signaling, nutrient sensing, oncogenic transformation, and metabolic regulation, where pS6 levels serve as a sensitive read-out of upstream kinase activity. The polyclonal format provides recognition of multiple epitopes surrounding the phospho-S235 site, supporting robust signal even when the modification is partial or dynamically regulated within experimental systems.