Anti-Rpn7 Antibody

This Antibodies.com polyclonal antibody specifically recognizes Rpn7, a non-ATPase subunit of the 19S regulatory particle lid subcomplex of the 26S proteasome in Saccharomyces cerevisiae. Rpn7 contributes to recognition of polyubiquitinated substrates and to lid assembly, making the antibody useful for studies of ubiquitin-dependent proteolysis and proteasome integrity. The reagent is supplied as 100 µl of liquid formulation, is unconjugated, and has been validated for western blot and immunoprecipitation workflows using yeast lysates. Storage recommendations call for keeping the vial refrigerated at 4 °C in working aliquots to limit freeze-thaw damage to the IgG preparation. Researchers investigating proteasome regulation, protein turnover, and UPS-linked signaling pathways deploy this reagent to immunoprecipitate Rpn7-containing lid complexes and to monitor steady-state proteasome levels by western blot.