Anti-RPL27A Antibody

Ribosomal protein L27a is a structural component of the 60S large ribosomal subunit, and the polyclonal antibody from Antibodies.com provides a robust tool for tracking its expression in cell and tissue samples. Raised in rabbit and supplied as an unconjugated 100 µl liquid aliquot, the reagent is suited to standard immunodetection workflows without requiring additional labeling steps.

Validated for Western blot and immunofluorescence applications, this Antibodies.com reagent recognizes endogenous RPL27A in human, mouse, and rat samples. RPL27A resides in the cytoplasm where it incorporates into assembling ribosomal subunits during ribosome biogenesis, and altered expression of the protein has been reported in studies of erythropoiesis, ribosomal stress, and in several cancer cell lines. As a member of the L10AE family of ribosomal proteins that carries a conserved KOW motif, RPL27A also serves as a widely used reference target in gene expression normalization panels. Researchers investigating translation regulation, ribosomal stress responses, and diseases linked to ribosomal protein dysfunction commonly use anti-RPL27A antibodies to assess 60S subunit integrity and to standardize loading across experimental conditions.

As a polyclonal reagent, the antibody binds multiple epitopes on RPL27A, supporting reliable detection across denatured and native protein preparations. The unconjugated format allows users to pair the primary with their preferred reporter-labeled secondary antibody for WB or IF, and the 100 µl volume is generally sufficient for multiple blotting membranes or coverslip-based imaging experiments when used at standard working dilutions.