Anti-RPL12 Antibody

Brand: Antibodies.com. This is a rabbit polyclonal primary antibody targeting RPL12, supplied as an unconjugated IgG in liquid form. The immunogen is a synthetic peptide derived from human RPL12, described in one source as an internal-region peptide and in another as amino acids 61-110.

Western blot (WB) is the application listed across the sources; an additional manufacturer source also lists IHC, IF and ELISA and states that the antibody is validated for WB, IHC, ICC/IF and ELISA. Reactivity with human and mouse samples is reported in every source, while rat reactivity appears in the additional manufacturer source. The antibody detects endogenous levels of total RPL12 protein, and that source reports no cross-reactivity.

Recommended dilutions given in that source are WB 1:500~1:1000 and ELISA 1:40000. The formulation is phosphate-buffered saline without Mg2+ and Ca2+, pH 7.4, with 150mM NaCl, 0.02% sodium azide and 50% glycerol, and the antibody is purified from rabbit serum by affinity chromatography using the immunizing peptide. It is shipped at 4°C; upon delivery, aliquot and store at -20°C, and avoid freeze/thaw cycles. One source lists a concentration of 1.0 mg/mL.