Anti-RPA32 / RPA2 Antibody

This mouse monoclonal antibody from Antibodies.com targets RPA32, the 32 kDa subunit of Replication Protein A (RPA), a heterotrimeric single-stranded DNA-binding complex essential for DNA replication, repair, and recombination.

Supplied as a 100 µl aliquot in an unconjugated IgG2b format, the reagent is validated for Western blot, immunoprecipitation, and immunocytochemistry workflows on human samples.

RPA32 is phosphorylated by ATM, ATR, and DNA-PKcs kinases at multiple sites during the DNA damage response, and antibodies against this subunit are routinely used to monitor replication stress, checkpoint activation, and genotoxic signaling in chromatin fractions and nuclear extracts.

Researchers studying DNA repair, homologous recombination, nucleotide excision repair, and S-phase progression commonly probe whole-cell lysates or fractionated chromatin for total or post-translationally modified RPA32 to map damage signaling events and replication fork integrity.