Anti-RPA2 Antibody

Replication Protein A2 (RPA2) forms the 32 kDa middle subunit of the heterotrimeric single-stranded DNA-binding complex that safeguards genome stability during DNA replication, repair, and recombination. Produced by Antibodies.com as an unconjugated rabbit polyclonal, this reagent recognizes endogenous RPA2 across human, mouse, and rat samples. Phosphorylation of RPA2 on serine and threonine sites through ATR and ATM kinases is a widely used molecular marker of replication fork stress and DNA damage signaling, supporting studies of checkpoint activation, S-phase progression, homologous recombination, and genotoxic stress responses.

Validated for western blot, immunohistochemistry, and immunofluorescence, the antibody enables detection of native RPA2 in cultured mammalian cells and tissue sections. Western blots show a band of approximately 32 kDa, consistent with the molecular weight of the RPA2 subunit. In immunofluorescence and immunohistochemistry, the polyclonal format offers signal amplification on fixed cells and paraffin-embedded tissue, compatible with both manual and automated staining workflows.

The product is supplied as 100 µl of antibody at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, ready for dilution into standard assay buffers. Each laboratory should titrate the reagent to identify the optimal working concentration for its specific protocol. Refrigerate on receipt and aliquot into smaller volumes for long-term storage to preserve binding activity across multiple experiments.