Anti-RPA2 Antibody

This rabbit polyclonal antibody from Antibodies.com targets RPA2, the 32 kDa subunit of the heterotrimeric Replication Protein A (RPA) complex that binds and stabilizes single-stranded DNA during chromosomal replication, nucleotide excision repair, and homologous recombination. The reagent is supplied as 50 µl of unconjugated IgG raised in rabbit, validated for Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation workflows across human and mouse sample types.

Researchers apply this reagent to study replication stress, the DNA damage response, and S-phase checkpoint signaling because RPA2 becomes phosphorylated on the N-terminus in response to genotoxic stress, and tracking total or modified RPA2 levels helps characterize pathway engagement in cancer cell lines, patient-derived tumor tissue, and engineered mouse models. The polyclonal format increases the likelihood of recognizing multiple epitopes along the subunit, which can be useful when probing denatured lysates in Western blot or fixed sections in IHC, and the unconjugated formulation allows pairing with secondary antibodies of the user's choice for chromogenic or fluorescence detection.

For storage and handling, keep the vial refrigerated, aliquot to avoid repeated freeze-thaw cycles, and protect from light to preserve antibody activity. The product is intended for research use only, supporting applications in vitro and in cell-based assays.