Anti-RPA2 Antibody

Antibodies.com offers a rabbit polyclonal primary antibody targeting Replication Protein A2 (RPA2), the 32 kDa subunit of the heterotrimeric single-stranded DNA-binding RPA complex that stabilizes exposed ssDNA during DNA replication, nucleotide excision repair, and homologous recombination. The unconjugated IgG reagent is raised in rabbit and was validated for reactivity against both human and mouse RPA2, supporting cross-species work on genome maintenance and the DNA damage response. Phosphorylation of RPA2 at sites such as Ser33 by ATR and DNA-PKcs is widely used in the field as a readout of replication stress and double-strand break signaling, so a polyclonal reagent that captures the native protein is valuable for probing checkpoint activation across multiple assay types, including western blot detection of total and phospho-RPA2, immunoprecipitation of RPA-containing complexes, immunofluorescence visualization of nuclear RPA foci following genotoxic treatment, and immunohistochemistry of formalin-fixed tissue sections. The reagent is supplied as 100 µl and is intended for research use only in basic and translational studies of DNA repair, cancer biology, and cell cycle checkpoint control.