Anti-RPA1 Antibody

Replication Protein A1 (RPA1) is the largest subunit of the heterotrimeric Replication Protein A complex, a single-stranded DNA-binding protein essential for DNA replication, nucleotide excision repair, homologous recombination, and the activation of the S-phase checkpoint following replication stress. RPA1 contributes multiple oligonucleotide/oligosaccharide-binding (OB) folds that engage ssDNA intermediates generated at stalled replication forks and during DNA damage processing, which is why it is commonly used as a marker for replication fork integrity and the DNA damage response in mammalian cells.

This mouse monoclonal reagent from Antibodies.com targets RPA1 and is provided as a purified antibody in 100 µl of buffer containing 0.1 M Tris-Glycine at pH 7.4, 150 mM NaCl, 50% glycerol, and 0.2% sodium azide as a preservative. The unconjugated antibody is validated for Western blot (WB), immunoprecipitation (IP), and immunocytochemistry (ICC) and has been confirmed to react with Human, Mouse, Rat, and Monkey samples, supporting cross-species work in standard cell lines and rodent tissue models.

Typical uses include monitoring chromatin-bound RPA pools after treatment with replication-stress inducers such as hydroxyurea or aphidicolin, tracking RPA recruitment to laser-induced DNA damage stripes, and probing the ATR–Chk1 signaling axis in cell-based assays. For chromatin fractionation experiments, IP enrichment of RPA1 allows downstream mass spectrometry or co-immunoprecipitation of interacting partners such as ATR, RAD51, or the MRE11–RAD50–NBS1 complex, providing a snapshot of repair complex assembly at stalled or damaged forks. For research use only.