Anti-RPA 32 kDa subunit Antibody

The RPA 32 kDa subunit (also called RPA2) forms the middle component of the heterotrimeric Replication Protein A complex that shields single-stranded DNA during replication and repair. Phosphorylation of this subunit on serine and threonine residues is a recognized marker of ongoing DNA damage response signaling, so antibodies raised against it are commonly used to probe replication stress and genotoxic exposure in human cells and tissue. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl aliquots and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human samples. Host species is rabbit, the antibody is polyclonal, and the format is unconjugated, allowing downstream labeling with secondary detection reagents of the researcher's choice. Researchers studying S-phase checkpoint activation, UV or IR-induced damage, and recombination pathways frequently include RPA 32 kDa subunit detection in their western blot panels alongside markers such as γH2AX and phospho-CHK1.