Anti-RPA 14 kDa subunit Antibody

This rabbit polyclonal antibody from Antibodies.com targets the 14 kDa subunit of Replication Protein A (RPA3), a single-stranded DNA-binding component essential for DNA replication, repair, and recombination in human cells. Supplied as an unconjugated reagent in a 100 µl volume, it is validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human samples, supporting studies of genome stability, replication stress, and the cellular DNA damage response.

Raised in rabbit, the polyclonal reagent recognizes the native RPA3 subunit as part of the heterotrimeric RPA complex. It enables detection of the small 14 kDa subunit by immunoblotting of whole-cell lysates and by chromogenic or fluorescent IHC staining of fixed tissue sections. Investigators working on nucleotide excision repair, homologous recombination, and checkpoint signaling routinely use antibodies against RPA subunits to monitor ssDNA exposure and replication fork integrity.

Provided unconjugated and free of carrier proteins, the antibody offers flexibility to conjugate it to HRP, biotin, or fluorophores of the user's choice. Storage recommendations and buffer composition details are supplied with the vial by Antibodies.com to preserve activity across multiple freeze-thaw cycles typical of long-term laboratory use.